Download and read online Microchip Capillary Electrophoresis in PDF and EPUB Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. "Microchip Capillary Electrophoresis: Methods and Protocols" provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.
Download and read online Capillary Electrophoresis in PDF and EPUB This book presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. It includes practical details which are hard to find elsewhere. The volume is intended for beginners in the field and provides an overview of the technique and a starting point for the exploration of the defined literature on different application topics.
Download and read online Capillary Electrophoresis of Proteins and Peptides in PDF and EPUB This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.
Download and read online Clinical Applications of Capillary Electrophoresis in PDF and EPUB This Methods in Molecular Biology book is an in-depth manual of Capillary Electrophoresis applications in important areas of clinical science, including clinical chemistry, hematology, disease-associated biomarker discovery, immunology and genetic analysis."
Download and read online Capillary Electrophoresis of Biomolecules in PDF and EPUB This book details key techniques used to investigate Capillary electrophoresis (CE). It focuses on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids.
Download and read online Chiral Separations in PDF and EPUB Prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as specific applications to substance classes and special compounds, with emphasis on high performance liquid chromatography and capillary electrophoresis techniques, but also including thin layer chromotographic, gas chromatographic, supercritical fluid chromatographic as well as recent electrochromotographic techniques.
Download and read online Microchip Capillary Electrophoresis in PDF and EPUB Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.
Download and read online Capillary Electrophoresis Guidebook in PDF and EPUB Capillary Electrophoresis Guidebook offers both newcomers and experienced research workers hands-on guidance to performing capillary electrophoresis. It provides sufficient practical advice to permit you to develop and optimize your own separations, along with extensive troubleshooting sections to overcome practical difficulties. The book contains operating instructions for standard commercially available instruments and includes guidelines for activities such as changing capillaries, method development, quantitative procedures, optimizing sensitivity, and the validation of methods. Review chapters written by leading experts discuss micellar electrokinetic capillary chromatography, capillary gel electrophoresis, advanced sampling techniques, and electrochromatography. Important application areas such as the analysis of proteins, peptides, amino acids, pharmaceuticals, chiral compounds, and nucleic acids are also treated.
Download and read online Metabolomics in PDF and EPUB Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.
Download and read online Clinical Applications of Capillary Electrophoresis in PDF and EPUB In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today.
Download and read online Microchip Capillary Electrophoresis Protocols in PDF and EPUB In this volume expert researchers in the field detail the operations of microchip capillary electrophoresis. Chapters focus on small molecule, biomolecule applications, various detection modes, and sample preparation approaches are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microchip Capillary Electrophoresis Protocol aids scientists in continuing to study microchip capillary electrophoresis.
Download and read online Connexin Methods and Protocols in PDF and EPUB An essential element in direct cell-cell communication between virtually all cell types in vertebrates, connexins have been recently linked to an increasing number of human genetic diseases. In Connexin Methods and Protocols, well established investigators describe in detail their best techniques for studying the cellular and molecular biology of connexins, as well as their physiological properties. These forefront methods are specifically designed to investigate the life of connexins from biosynthesis to assembly into gap junction channels, and also include powerful assays to perform functional studies on these routes of cell-cell communication. To ensure experimental success, each time-tested technique contains detailed step-by-step instructions, as well as tips on how to avoid pitfalls. Also discussed are the many specific theoretical aspects of connexin biology and physiology. Cutting-edge and highly practical, Connexin Methods and Protocols offers laboratory researchers a comprehensive collection of proven methods for studying the role of connexins in intercellular communication and human genetic disorders, as well as finding promising new therapeutics for those disorders.
Download and read online Neurogenetics in PDF and EPUB An international panel of recognized academic physicians, researchers, and clinical laboratory diagnosticians describe their best methods for characterizing neurologically relevant genes, their mutations, and their proteins. Providing detailed step-by-step instructions to assure successful experimental results, these experts cover the key methods for mutation detection and screening, including discussions of quantitative PCR, trinucleotide repeat detection, sequence-based mutation detection, fluorescence in situ hybridization (FISH), in vitro protein expression systems, and studies of protein expression. Understand the functional consequences of neurologically relevant gene mutations Enjoy a comprehensive collection of techniques for mutation detection and screening.
Download and read online The Protein Protocols Handbook in PDF and EPUB In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.
Download and read online Pichia Protocols in PDF and EPUB This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.